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《医学前沿(英文)》 2022年 第16卷 第3期 页码 378-388 doi: 10.1007/s11684-021-0840-y
关键词: severe Mycoplasma pneumoniae pneumonia children proteomics Fc fragment of the IgG-binding protein mechanistic target of rapamycin kinase inhibitor
Chloride binding and time-dependent surface chloride content models for fly ash concrete
S. MUTHULINGAM,B. N. RAO
《结构与土木工程前沿(英文)》 2016年 第10卷 第1期 页码 112-120 doi: 10.1007/s11709-015-0322-x
关键词: binding isotherms chloride ingress concrete fly ash surface chloride content
reduces IgE binding ability of allergenic egg white proteins
Sen LI, Marina OFFENGENDEN, Michael G. GÄNZLE, Jianping WU
《农业科学与工程前沿(英文)》 2018年 第5卷 第3期 页码 373-381 doi: 10.15302/J-FASE-2018210
Egg white proteins are one of the major allergens. The objective of this study was to investigate the effect of Aspergillus oryzae cultivation on IgE binding ability of egg white proteins. Effect of A. oryzae on egg white proteins was determined using ninhydrin method, SDS-PAGE, ELISA, fluorescence FITC labeling, MALDI-TOF-MS and LC-MS/MS analysis. Adding mycelium of A. oryzae ATCC 1011 and 16868 substantially reduced the IgE binding ability of acidified egg white after 24 h incubation. The binding capacity of egg white proteins to IgE in plasma from four egg allergy patients was almost completely lost after incubation with mycelium of ATCC 16868. Results from SDS-PAGE, free amino acid analysis, MALDI-TOF-MS and LC-MS/MS indicated that there was no substantial protein degradation during incubation. Therefore, the reduction of IgE binding ability of egg white proteins during A. oryzae treatment was probably due to a loss of ~1700 Da mass including a fragment of the ovomucoid N terminus.
关键词: Aspergillus oryzae egg allergy egg white proteins IgE-binding ability ovomucoid
Yuan ZHANG,Yan ZHANG,Tao YU
《环境科学与工程前沿(英文)》 2014年 第8卷 第5期 页码 666-674 doi: 10.1007/s11783-013-0608-y
关键词: binding ability dissolved organic matters fluorescence quenching complex capacity Taihu Lake
Repression of CDKN2C caused by PML/RARα binding promotes the proliferation and differentiation block
null
《医学前沿(英文)》 2016年 第10卷 第4期 页码 420-429 doi: 10.1007/s11684-016-0478-3
Inappropriate cell proliferation during oncogenesis is often accompanied by inactivation of components involved in the cell cycle machinery. Here, we report that cyclin-dependent kinase inhibitor 2C (CDKN2C) as a member of the cyclin-dependent kinase inhibitors is a target of the PML/RARα oncofusion protein in leukemogenesis of acute promyelocytic leukemia (APL). We found that CDKN2C was markedly downregulated in APL blasts compared with normal promyelocytes. Chromatin immunoprecipitation combined with quantitative polymerase chain reaction demonstrated that PML/RARα directly bound to the CDKN2C promoter in the APL patient-derived cell line NB4. Luciferase assays indicated that PML/RARα inhibited the CDKN2C promoter activity in a dose-dependent manner. Furthermore, all-trans retinoic acid treatment induced CDKN2C expression by releasing the PML/RARα binding on chromatin in NB4 cells. Functional studies showed that ectopic expression of CDKN2C induced a cell cycle arrest at the G0/G1 phase and a partial differentiation in NB4 cells. Finally, the transcriptional regulation of CDKN2C was validated in primary APL patient samples. Collectively, this study highlights the importance of CDKN2C inactivation in the abnormal cell cycle progression and differentiation block of APL cells and may provide new insights into the study of pathogenesis and targeted therapy of APL.
关键词: CDKN2C acute promyelocytic leukemia cell cycle arrest differentiation
Jingde LUAN,Rundong LI,Zhihui ZHANG,Yanlong LI,Yun ZHAO
《环境科学与工程前沿(英文)》 2014年 第8卷 第6期 页码 871-876 doi: 10.1007/s11783-013-0621-1
关键词: sludge incineration heavy metals inner speciation redistribution binding energy
null
《医学前沿(英文)》 2014年 第8卷 第2期 页码 217-226 doi: 10.1007/s11684-014-0326-2
This study was designed to investigate the contribution of miRNA-122-binding site polymorphism at the IL-1A gene and its multiplicative interactions with hepatitis B virus (HBV) mutations in the risk of hepatocellular carcinoma (HCC). A total of 1021 healthy controls, 302 HBV surface antigen (HBsAg) seroclearance subjects, and 2011 HBsAg-positive subjects (including 1021 HCC patients) were enrolled in this study. Quantitative PCR was used to genotype rs3783553. HBV mutations were determined by direct sequencing. Multivariate logistic regression analyses were performed to test the associations of rs3783553, mutations, and their interactions with the risk of HCC. No significant association was found between rs3783553 and the risk of HCC among healthy controls, HBsAg seroclearance subjects, HBsAg-positive subjects without HCC, and all controls. Additionally, rs3783553 was not significantly associated with chronic HBV infection, liver cirrhosis, HBV e antigen seroconversion, abnormal alanine aminotransferase, and high viral load (>104 copies/ml). However, the TTCA insertion allele of rs3783553 was significantly associated with an increased frequency of HBV C7A mutation compared with homozygous TTCA deletion carriers [(del/ins+ ins/ins) vs. del/del, adjusted odds ratio (OR)=1.48, 95% confidence interval (CI)=1.09-2.02, P=0.013]. Multiplicative interaction of rs3783553 with HBV preS deletion significantly reduced the risk of HCC in males, with an adjusted OR of 0.64 (95% CI=0.42-0.98; P=0.041) after age and HBV genotype were adjusted. Although rs3783553 did not significantly affect genetic susceptibility to HBV-related HCC, its variant allele may predispose the host to selecting HBV C7A mutation during evolution and significantly reduce the risk of HCC caused by HBV preS deletion. This study provides an insight into the complex host-virus interaction in HBV-induced hepatocarcinogenesis and is helpful in determining HBsAg-positive subjects who are likely to develop HCC.
关键词: hepatocellular carcinoma (HCC) interaction miRNA-122-binding site IL-1A rs3783553 hepatitis B virus (HBV) mutations
Ying Cui, Feng Tan, Yan Wang, Suyu Ren, Jingwen Chen
《环境科学与工程前沿(英文)》 2020年 第14卷 第6期 doi: 10.1007/s11783-020-1290-5
关键词: Diffusive gradients in thin films Molecularly imprinted polymers Selective uptake Antibiotics Passive sampling
Approaching the binding between Cu(II) and aerobic granules by a modified titration and µ-XRF
Hongwei LUO,Longfei WANG,Zhonghua TONG,Hanqing YU,Guoping SHENG
《环境科学与工程前沿(英文)》 2016年 第10卷 第2期 页码 362-367 doi: 10.1007/s11783-015-0803-0
关键词: aerobic granules Cu(II) modified titration µ-XRF analysis
Atomistic characterization of binding modes and affinity of peptide inhibitors to amyloid-
Fufeng LIU,Wenjie DU,Yan SUN,Jie ZHENG,Xiaoyan DONG
《化学科学与工程前沿(英文)》 2014年 第8卷 第4期 页码 433-444 doi: 10.1007/s11705-014-1454-6
关键词: Alzheimer’s disease amyloid β-protein peptide inhibitors protein-protein interaction molecular dynamics simulation
Molecular diffusion in ternary poly(vinyl alcohol) solutions
《化学科学与工程前沿(英文)》 2022年 第16卷 第6期 页码 1003-1016 doi: 10.1007/s11705-021-2121-3
关键词: fluorescence correlation spectroscopy poly(vinyl alcohol) anomalous diffusion crowding effects dynamic light scattering binding effects rhodamine B
Biosorption of Cu(II) to extracellular polymeric substances (EPS) from
Xiangliang PAN, Jing LIU, Wenjuan SONG, Daoyong ZHANG
《环境科学与工程前沿(英文)》 2012年 第6卷 第4期 页码 493-497 doi: 10.1007/s11783-012-0416-9
关键词: biosorption conditional binding constant extracellular polymeric substances (EPS) fluorescence quenching
Regulation of NLR stability in plant immunity
Tao WANG, Jiaxin LI, Qian-Hua SHEN
《农业科学与工程前沿(英文)》 2019年 第6卷 第2期 页码 97-104 doi: 10.15302/J-FASE-2018248
Plant nucleotide binding domain and leucine-rich repeat (NLR) receptors recognize pathogen effectors directly or indirectly and mediate innate immune responses. NLR-mediated immunity also has direct impacts on plant growth and development, as well as yield and survival. The levels of NLR proteins are therefore intricately controlled in plants to balance defense responses and other processes. In recent years, the ubiquitination-26S proteasome system and the HSP90 chaperones have emerged as having key functions in the regulation of NLR stability. The N-end rule pathway of protein degradation is also directly linked to NLR stability. Recent progress in the regulation of NLR stability and turnover is summarized here, focusing on the key components and pathways.
关键词: E3 ubiquitin ligase degradation nucleotide-binding leucine-rich repeat receptor plant immunity proteasome protein stability ubiquitination
Effects of resistin on insulin signaling in endothelial cells
Zhizhen LI, Fangping LI, Jianhong YE, Li YAN, Zuzhi FU
《医学前沿(英文)》 2009年 第3卷 第2期 页码 136-140 doi: 10.1007/s11684-009-0029-2
关键词: resistin endothelium nitric oxide endothelial nitric oxide synthase Akt-binding protein mouse
《化学科学与工程前沿(英文)》 2022年 第16卷 第7期 页码 1060-1078 doi: 10.1007/s11705-021-2117-z
关键词: anchoring groups adsorption behaviour dye-binding strength squaraine dyes dye-sensitized solar cells
标题 作者 时间 类型 操作
Proteomics study of Mycoplasma pneumoniae pneumonia reveals the Fc fragment of the IgG-binding protein
期刊论文
Chloride binding and time-dependent surface chloride content models for fly ash concrete
S. MUTHULINGAM,B. N. RAO
期刊论文
reduces IgE binding ability of allergenic egg white proteins
Sen LI, Marina OFFENGENDEN, Michael G. GÄNZLE, Jianping WU
期刊论文
Quantitative characterization of Cu binding potential of dissolved organic matter (DOM) in sediment from
Yuan ZHANG,Yan ZHANG,Tao YU
期刊论文
Repression of CDKN2C caused by PML/RARα binding promotes the proliferation and differentiation block
null
期刊论文
Speciation evolutions of target metals (Cd, Pb) influenced by chlorine and sulfur during sewage sludge incineration
Jingde LUAN,Rundong LI,Zhihui ZHANG,Yanlong LI,Yun ZHAO
期刊论文
Association of miRNA-122-binding site polymorphism at the interleukin-1 α gene and its interaction with
null
期刊论文
Diffusive gradients in thin films using molecularly imprinted polymer binding gels for in situ measurements
Ying Cui, Feng Tan, Yan Wang, Suyu Ren, Jingwen Chen
期刊论文
Approaching the binding between Cu(II) and aerobic granules by a modified titration and µ-XRF
Hongwei LUO,Longfei WANG,Zhonghua TONG,Hanqing YU,Guoping SHENG
期刊论文
Atomistic characterization of binding modes and affinity of peptide inhibitors to amyloid-
Fufeng LIU,Wenjie DU,Yan SUN,Jie ZHENG,Xiaoyan DONG
期刊论文
Biosorption of Cu(II) to extracellular polymeric substances (EPS) from
Xiangliang PAN, Jing LIU, Wenjuan SONG, Daoyong ZHANG
期刊论文
Effects of resistin on insulin signaling in endothelial cells
Zhizhen LI, Fangping LI, Jianhong YE, Li YAN, Zuzhi FU
期刊论文